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文献和实验Gene Transfer Using Targeted Filamentous Bacteriophage
transfer and subsequent protein expression is measured using a reporter gene such as green fluorescent protein (GFP), neomycin phosphotransferase, or β-galactosidase. In fact, any gene with an appropriate mammalian transcriptional promoter
Pollen Development in Anthers of Arabidopsis
in Petri dishes on a layer of sterilized superfine grade Perlite pre-wetted with Gamborg's B5 Medium (see Growing Arabidopsis protocol). 2. Incubate in a growth chamber at 19-23°C under cool white fluorescent light (1500 to 2200 lux).
by the addition of polyethylene glycol and collected by centrifugation. The DNA pellet is resuspended in a buffer containing cesium chloride and ethidium bromide, which is loaded into polyallomer tubes and subjected to ultracentrifugation overnight. The ethidium
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