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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
tert-Butyl6-bromo-4H-spirobenzod1,3dioxine-2,4'-piperidine-1'-carboxylate
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
895525-73-2
- 规格:
10mg/100mg/2724mg
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of 0.3% SDS). Mix by gentle pipetting. Shake gently for 1 h at 37°C. 15. Add 50 µL of 20% Triton X-100 (to a final concentration of 1.8%). Shake gently for 1 h at 37°C. 16. Add 1200 units of restriction enzyme (i.e., 12 µL
Establishment of Stable Transfectant of CHO Lec Cells
mannose type, but differ in the number of mannoses, ranging from Man9 to Man5 . O-glycosylation is homogenous, with only a single GalNAc residue attached per site. When cultured in the presence of the alpha-glucosidase I inhibitor N-butyl
Establishment of Stable Transfectant of CHO Lec Cells
. When cultured in the presence of the alpha-glucosidase I inhibitor N-butyl-deoxynojirimycin (NB-DNJ), glycoproteins produced in CHO Lec 3.2.8.1 cells are almost completely susceptible to Endo H digestion (Davis, 1995; Ikemizu, 1999). Endo H cleaves chitobiose
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