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- 文献和实验
- 技术资料
- 英文名:
2-2-(1,1'-Biphenyl-4-yloxy)ethylpiperidinehydrochloride
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
1219963-72-0
- 规格:
10mg///100mg///200mg
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文献和实验Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
Two dimensional peptide mapping
from the protease adsorbing onto the filter. 3. Aspirate the liquid. Wash the membrane extensively with H2O (5 X 1ml). Then wash with freshly-made 0.05 M NH4HCO3 once or twice. 4. Digestion: Add 150 -200 microliter fresh 0.05 M NH4HCO3
, if necessary by blowing. On ice, add 50 ml the Master mix.Running the cycles:Transfer the tubes directly from ice to the prewarmed 94 C block on the Robocycler and run the following cycles:94 C for 3 min 1 x94 C for 30 s; Tann.* for 1 min; 72 C for 1 min-1 min
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