(amino-5-bromo-3-methoxybenzoi

Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌

of the chromogenic substrate 5-bromo-4-chloro-3-indolyl-beta-D-galacoside (X-gal). However insertion of a fragment of foreign DNA into the polycloning site of the plasmid almost invariably results in production of an amino-terminal fragment that is not capable

妊娠期的内分泌特点

继续保持到分娩。妊娠早期hCG的主要功能是维持卵巢的黄体分泌孕酮以维持早期胚胎发育的需要；它在胎儿-胎盘复合体中还促进类固醇激素的生物合成。（二）人类胎盘催乳素人类胎盘催乳素（human placenta lactogen，hPL）也称人类绒毛膜促生长催乳素（human chorionic somatamammotropin，hCS），为单链多肽类激素，其分子量为21600u，由190个氨基酸残基合成，含有两条链内二硫键。人胎盘催乳素与人生长激素有160个相同的氨基酸残基序列，因此二者在免疫

Methods for use with the mTn-3xHA/lacZ-mutagenized library

for expression following transformation). Obtain 50,000 colonies for each pool. Elute colonies from plates in LB; make a -70o C stock of this eluate. Dilute eluate into LB plus antibiotic to give a culture with an almost saturated density. Grow at 37o C