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- 文献和实验
- 技术资料
- 英文名:
3-Amino-5-fluorobenzeneboronic acid pinacol ester
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
710348-95-1
- 规格:
10mg///100mg///200mg
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文献和实验Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
“bait”-peptides: Biotinylated histone peptides with specific modifications can be chemically synthesized or obtained commercially. Peptides should be HPLC purified to >80% purity, aliquoted, lyophilized, and stored dry at -80°C. (See comment 1).
Synthesis and Probing of Membrane-bound Peptide Arrays
upon cleavage of the peptide. We have successfully added biotin via its in situ formed HOBt-ester (normal activation procedure) or fluorescein via its isothiocyanate (FITC) dissolved in DMF. After the final amino acid elongation cycle
of trifluoroacetic acid (TFA) cleavage cocktail1 (e.g. 95% aq. TFA, see discussion) to the scintillation glass vial and cap tightly. Shake for 10 minutes to 2 hours at room temperature (see discussion). 3) Collect the TFA cleavage solution by filtering
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