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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
N4-(3-Chloro-4-fluorophenyl)-7-methoxyquinazoline-4,6-diamine
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
179552-75-1
- 规格:
10mg/100mg/9184mg
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文献和实验containing chloramphenicol (Cm, 20 μg/ml)/streptomycin (Stp, 75 μg/ml) and purified using the Large construct kit (Qiagen, Valencia, CA, USA). PCR PCR reactions using oligonucleotides carrying HAs were performed using
Purification of GST fusion proteins in E.coli GST融合蛋白纯化(方法四)筛选表达
proteins).-Note: Crude sonicates can be screened for the relative level of expression of GSTfugion proteins using the GST substrates CDNB (1-chloro-2,4-dinitrobenzene).Centrifuge in a microcentrifuge for 5 min to remove insoluble materials.Save a10 μl
using the GST substrates CDNB (1-chloro-2,4-dinitrobenzene).Centrifuge in a microcentrifuge for 5 min to remove insoluble materials.Save a10 μl aliquots of the insoluble material for analysis by SDS-PAGE.Transfer the supernatants to fresh tubes,Add 20 μl
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