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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
(E)-Ethyl3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)acrylate
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
1009307-13-4
- 规格:
10mg///100mg///200mg
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文献和实验Transfection of E. coli with M13 DNA
In recent years several techniques have been described for the introduction of DNA molecules into strains of E. coli by transformation or transfection. These are based on the findings of Mandel and Higa (1 ) who demonstrated that incubation
E-Z 96® M13 Isolation Vacuum Manifold Protocol
the E-Z 96 DNA Plate back on the manifold and apply the vacuum for another 5 minutes. Repeat the tapping step from step 11 to completely remove any remaining liquid. 13. Optional: Place the E-Z® 96 DNA Plate into a vacuum oven preset at 60°C
in cold calcium chloride resulted in uptake of virus and that a transient heat shock of the mixture greatly enhanced the efficiency of transformation. Subsequently, this method was used to introduce a variety of circular and linear DNAs into strains of E
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