(2-(naphthalen-2-yl)phenyl)-1,

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

) and Cs2 CO3 under a nitrogen atmosphere. Conversion to the O 6 ?(benzotriazol?1?yl) ethers occurs within about 10 min for inosine, and within about 60 min for guanosine and 2??deoxyguanosine. Then, for reaction with alcohols, the reaction mixture

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

‐(1‐(2‐((2‐Azidoethyl)(2‐(bis(4‐methoxyphenyl)(phenyl)methoxy)ethyl)amino)‐2‐oxoethyl)‐N 4 ‐(benzoyl)cytosin‐1‐yl) (19 ; Basic Protocol 3) Tetrahydrofuran (THF), anhydrous, ≥99.9% pure

Nat Methods：陈玲玲/杨力/李劲松等开发新技术，实现环状 RNA 功能性筛选和研究

获得异种移植物。满足 CDCscreen score≥2、有效 gRNAs≥2 且倍数变化≤0.5 条件的共有 79 个 circRNA，其中 8 个与 HT29、HeLa、293FT 及 H9 细胞系中进行功能缺失（loss-of-function，LOF）表型体外筛选结果重叠。CircFAM120A 是这些重叠的候选基因之一。上述结果验证了 RfxCas13d/BSJ-gRNA 可用于人细胞系功能性 circRNA 筛选。 CircFAM120A 的体内体外筛选（图源：Nature