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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
N-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)propionamide
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
2246698-15-5
- 规格:
10mg///100mg///200mg
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文献和实验The separation and determination of O -phosphoamino acids has been carried out by thin-layer chromatography (1 –8 , thin-layer electrophoresis (1 ,6 ,7 ,9 –14 ), gel electrophoresis (15 ), amino acid analyzer (16 –18 ), high
Assay for ß-galactosidase in E. Coli
, isopropyl-ß-thiogalactoside (IPTG), phenyl-ß-galactoside (PBG) and glucose (GLU). We will use a non-biological (artificial) substrate for the enzyme, Ortho-nitrophenyl-ß-galactoside (ONPG). In the presence of ß-galactosidase ONPG is converted
(1,2-dioxetane-3,2'-(5'-chloro)tricyclo[3,3.1.13.7 ]decan}-4-yl) phenyl phosphate, more commonly known as CSPD (Cat. No. 1655 884). Blot Preparation: 1. Denature DNA in gel by soaking in 1X denaturation buffer 30-60 min. 2. Neutralize by soaking gel in 1X
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