(3-Ethoxy-4-methoxy-phenyl)eth
yl-amine HCl labelled:1398065-60-5;unlabelled:403842-03-5 **科研现货速达** ≥4204% HPLC 随货提供COA、HPLC谱图、MS谱图，NMR图谱等，详细联系客服。用于LC-MS/MS定量分析，代谢组学、质谱，药代动力学等- 询价
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
D7-2- (3-Ethoxy-4-methoxy-phenyl)ethyl-amine HCl
- 库存：
999
- 供应商：
智溯科ZSK
- CAS号：
labelled:1398065-60-5;unlabelled:403842-03-5
- 规格：
10mg///100mg///200mg
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文献和实验Preparation of probes for in situ hybridization
acitity (60-80% of the free amine will react), while 50 µg will yield a lower activity (5-20% of the free amine will be conjugated). Labelling is initiated by adding 30 µl of DMSO containing the activated fluorophore. In principle, any amine reactive
Preparation of probes for in situ hybridization
in 70 µl of 0.1 M NaHCO3 buffer, pH 8.8. Different amounts of material will result in different specific activities of the probe. 5-10 µg of nucleic acid will yield a high specific acitity (60-80% of the free amine will react), while 50 µg will yield
Southern Blotting: DNA Transfer
1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (small gels), 7' (large gels) 2. Denaturation: Wash gel in (0.5 M NaOH, 1.5 M NaCl) for 20' (small) to 30' (large). 3. Neutralization: Wash in (0.5 M Tris pH 7.0, 3.0 M NaCl) for 20
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