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细胞污染识别与处理全攻略:5 种常见类型+关键误区abinScience 体内级抗体,年度锁价,低至 1200五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 英文名:
D7-2- (3-Ethoxy-4-methoxy-phenyl)ethyl-amine HCl
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:1398065-60-5;unlabelled:403842-03-5
- 规格:
10mg///100mg///200mg
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文献和实验Preparation of probes for in situ hybridization
acitity (60-80% of the free amine will react), while 50 µg will yield a lower activity (5-20% of the free amine will be conjugated). Labelling is initiated by adding 30 µl of DMSO containing the activated fluorophore. In principle, any amine reactive
Preparation of probes for in situ hybridization
in 70 µl of 0.1 M NaHCO3 buffer, pH 8.8. Different amounts of material will result in different specific activities of the probe. 5-10 µg of nucleic acid will yield a high specific acitity (60-80% of the free amine will react), while 50 µg will yield
Southern Blotting: DNA Transfer
1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (small gels), 7' (large gels) 2. Denaturation: Wash gel in (0.5 M NaOH, 1.5 M NaCl) for 20' (small) to 30' (large). 3. Neutralization: Wash in (0.5 M Tris pH 7.0, 3.0 M NaCl) for 20
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