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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Nitrobenzene D5 1000 µg/mL in Isooctane
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:4165-60-0
- 规格:
10mg///100mg///200mg
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文献和实验Solutions for Southern Hybridization and Development Hybridization
to ~50-70° C; the sol'n remains turbid. Make ~1 hour in advance. Store hybridization solution at 4° C in a bottle or at -20° C in 50 ml conical tubes. Note: Prehybridization solution is the hybridization solution without the labelled probe.
RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes
probe 10x Taq Buffer 2.5 µl DIG DNA Labeling Mix, 10 x conc. 2.5 µl Primer * (10 pmol/ul) 1.0 µl Purified PCR product (at least 200 µg/ml) 4.0 µl Taq Polymerase 2 units
Southern Blotting(Fred Hutchinson Cancer Research Cente
step can be skipped without increasing background. If the the half-life of your working CSPD solution begins to decrease rapidly in storage, try using a new storage container (e.g., 50-ml Falcon tube); storage bottles reused many times apparently
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