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- 文献和实验
- 技术资料
- 英文名:
(S)-tert-Butyl3-(methylsulfonyloxy)piperidine-1-carboxylate
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
940890-90-4
- 规格:
10mg///100mg///200mg
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文献和实验microliters 1/10 diluted piperidine and store on ice. 4、To the A+C tube, add 1.0 microliter 30% NaOH. Incubate 90 degrees for 15 min. This step works best if performed in a PCR tube and incubated in a PCR instrument with a heated lid to prevent evaporation
of water to prepare a 1M piperidine solution. Place the tube containing the formic acid reaction on ice, add all 150 μl of the diluted (1M) piperidine solution and incubate for 30 min at 90C. Place this reaction on ice for 5min, add 1ml
, add all 150 ul of the diluted (1M) piperidine solution and incubate for 30 min at 90℃. 5、Place this reaction on ice for 5min, add 1ml of n-butanol and vortex. 6、Centrifuge for 2mi8n at high speed to pellet the DNA. Remove the supernatant and add
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