(D17;1-15N-4-Amino-2,2,6,6-tet

Hints and precautions for the care, feeding and breeding of Neurospora

with a glass spreader, use a separate spreader for each cross. Alcohol flaming is inadequate to kill all adhering ascospores (Newmeyer et al. 1971 N.N. 18:13).   LAB PRACTICE, HOUSEKEEPING 15. Avoid drafts. Make transfers in a draft-free room. Don''t

Replication timing by density transfer

C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered

基于极性与非极性氨基酸的“二元组图” 进行蛋白质设计

)。在 β 片层库中，转角区域的设计则基于已知结构的天然蛋白质中氨基酸残基组合的 “转角可能性”（见注 2’；参考文献 [22])。 ( 2 ) 转角区域的序列中可引入限制性位点。这在基因扩增中常常很有用（见 9.2.3；参考文献 [8])。 ( 3 ) 固定区域的长度应满足序列特异性退火的需要。重叠 10~15 个碱基的寡核苷酸对通常用于退火反应。亦可通过固定转角区域前后密码子中的 1 或 2 个碱基来帮助退火。例如，合成寡核苷酸链 (5， - N AN - N TN - N TN - N