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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
4-Cyano-4-methyl-piperidine-1-carboxylic acid benzyl ester
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
1372534-37-6
- 规格:
10mg///100mg///200mg
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
fresh 10 mg/ml stock in DMSO.Need 2.5 mg per 50 mg of tubulin. 8.Potassium glutamate,1 M.Prepare 1 M glutamic acid and titrate with KOH to pH 6.9. 9.Injection Buffer: 50 mM potassium glutamate,0.5 mM MgCl2,pH 6.5-6.7.Prepare 10 ml.Keep ~5 ml at 37℃
?cyanoethylthio group is effected by treatment with 2,4,6?triisopropybenzenesulfonyl chloride and triethylamine to give a 6?(2,4,6?triisopropylbenzenesulfonic acid) ester intermediate. Reaction of this key intermediate with 3?mercaptoproprionitrile
Labeling Tubulin and Quantifying Labeling Stoichiometry
5 (and-6) carboxyfluorescein succinimidyl ester ( Molecular Probes C-1311) Oregon Green 488 carboxylic acid, succinimidyl ester 5-isomer ( Molecular Probes O-6147) 5 (and-6) carboxytetramethylrhodamine succinimidyl ester ( Molecular Probes C-1171
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