CheKine™ 活性氧（ROS）含量检测试剂盒（荧光酶标仪

CheKine™ 活性氧（ROS）含量检测试剂盒（荧光酶标仪法）

货号：KTB1912 | 精准定量细胞氧化应激水平

专为科研场景设计的荧光法检测试剂盒，适用于动植物组织与细胞样本中活性氧（ROS）的定量分析。利用DCFH-DA探针特异性氧化反应机制，荧光强度与ROS含量线性正相关。

核心检测原理

活性氧（ROS）作为氧代谢副产物，其积累可导致细胞氧化损伤。本试剂盒采用DCFH-DA探针，在细胞内被ROS氧化生成强荧光产物DCF。通过荧光酶标仪检测激发波长502nm/发射波长530nm信号强度，实现ROS水平精准量化。

标准化试剂盒组分

• • Reagent I
• • Reagent II

关键实验流程控制点

操作前必读：

• • 实验环境：全程穿戴防护装备（手套/防护镜），刺激性与毒性物质需在通风橱内操作
• • 样本前处理：推荐BCA法蛋白定量实现样本均一化
• • 标准曲线：需用户自行建立样本特异性标准曲线
• • 预实验：正式实验前强烈建议预实验验证条件

规格与交付信息

稳定性保障方案

• • 储存条件：-20℃避光保存
• • 有效期：6个月
• • 运输方式：蓝冰冰链运输维持试剂活性

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Reactive Oxygen Species (ROS) Assay Kit (Fluorescence Microplate Reader Method)

Catalog Number: KTB1912 | Accurate Quantification of Cellular Oxidative Stress Levels

A fluorescence-based assay kit specifically designed for research applications, enabling quantitative analysis of reactive oxygen species (ROS) in animal/plant tissues and cell samples. Utilizes the specific oxidation mechanism of DCFH-DA probe, with fluorescence intensity linearly proportional to ROS content.

Core Detection Principle

ROS, as byproducts of oxygen metabolism, accumulate and cause cellular oxidative damage. This kit employs DCFH-DA probe, which is oxidized by intracellular ROS to generate highly fluorescent DCF. ROS levels are precisely quantified by measuring fluorescence intensity at excitation/emission wavelengths of 502nm/530nm using a microplate reader.

Standardized Kit Components

• • Reagent I
• • Reagent II

Critical Experimental Process Controls

Mandatory pre-reading:

• • Experimental environment: Wear full PPE (gloves/goggles) throughout; handle irritant/toxic substances in fume hood
• • Sample pretreatment: Recommend BCA protein quantification for sample normalization
• • Standard curve: Users must establish sample-specific standard curves
• • Pilot experiment: Strongly recommend preliminary validation before formal experiments

Specifications & Delivery Information

Stability Guarantee

• • Storage conditions: -20℃ protected from light
• • Shelf life: 6 months
• • Shipping method: Blue ice chain transport to maintain reagent activity

Note: Above information may change due to product optimization. Please consult Yakeyin Company for details.