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文献和实验Direct Fluorochrome-Labeled DNA Probes for Direct Fluorescent In Situ Hybridization to Chromosomes
and the system is used mostly to detect middle- to high-copy number (Fig. 1 ) or pooled probes, although antibody amplification of some fluorochrome labels is possible ( 1 ), and then the method must be evaluated in comparison with digoxigenin (chapter 27 ) or biotin
in 2X SSC for 5 minutes each at 42°C; If a biotin-labelled probe (e.g. chromosome paint) is used, a detection step has to be included; Block in 4X SSC/0.1% Tween/5% BSA (Gibco) for 15 minutes at RT; Incubate in fluorescently
here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions. Curr. Protoc. Neurosci. 59:1.20.1?1.20.18. © 2012 by John Wiley & Sons
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