- ￥892.18
- BioLegend
- 100212
- 美国
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Alexa Fluor® 488 anti-mouse CD3
- 抗体英文名：
Alexa Fluor® 488 anti-mouse CD3
- 供应商：
上海善然生物科技有限公司
- 保存条件：
2-8℃
- 规格：
25ug
Alexa Fluor® 488 anti-mouse CD3 Antibody
17A2
Catalog# / Size 100212 / 25 µg
Clone
Regulatory Status RUO
Other Names T cell antigen receptor complex, T3
Isotype Rat IgG2b, κ
Description CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells,
and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ
chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in
TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen
complex
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文献和实验分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
1) Resuspend the Dynabeads Mouse T-Activator CD3/CD28 in the vial. 2) Transfer the desired volume of Dynabeads to a tube. 3) Add an equal volume of Buffer, or at least 1 ml, and mix (vortex for 5 seconds
using Dynabeads® CD3⁄CD28 CTS™. Following separation, the CD3 T cells are cultured in the presence of the beads. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads will provide both the primary and co-stimulatory signals
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