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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PE/Cyanine5 anti-mouse I-A/I-E
- 抗体英文名:
PE/Cyanine5 anti-mouse I-A/I-E
- 供应商:
上海善然生物科技有限公司
- 保存条件:
2-8℃
- 规格:
100ug
PE/Cyanine5 anti-mouse I-A/I-E Antibody
M5/114.15.2
Catalog# / Size
107612 / 100 µg
Clone
Regulatory Status RUO
Other Names MHC class II
Isotype Rat IgG2b, κ
Description These class II molecules are expressed on antigen presenting cells (including B cells) and a
subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells
expressing CD3/TCR and CD4 proteins.
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文献和实验To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
cut paraffin sections and deparaffinize with xylene and graded EtOH into PBS. Block slides with 1/20x serum/PBS (use the appropriate blocking serum, i.e. same as 1º Ab), 10'. Wash in PBS 2' x3. Add digoxigenin labeled 1º antiobody.
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