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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
S-Adenosyl-L-methionine
- 库存:
99
- 供应商:
联硕生物
- CAS号:
29908-03-0
- 规格:
1ml
纯度:10mM in DMSO
保存温度:-80℃(运输条件:冰袋低温运输)
英文名称:S-Adenosyl-L-methionine
分子式:C15H22N6O5S
KRTHWPQUYLJSDBEOFAMNXCIVGZ
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文献和实验Cell Lysis/Western/IPSteven Finkbeiner,Departments of Neurology and Physiology,UCSFcompiled by 6/95 by AJS from AB)Fresh 1X LysisFresh 1X LysisFinal5ml10ml2X Lysis 2.5 ml5mlNaCl0.25M0.25ml of 5M0.5 ml of 5MNa3VO40.1mM25l (μl) of 200 mM5l (μl) of 200
Yeast Chromatin Immunoprecipitation (ChIP)
(425-600 micron diameter) 3. Lock every Marsh tube to ensure the tubes don’t open during lysis 4. Lyse cells in the Breeden Lab FastPrep vortex 3 x 40 sec, with 40 sec pauses between runs, at level 4.5 M/s (A2-124 cold room) 5. Remove locks
Yeast Chromatin Immunoprecipitation (ChIP)
from each 200μl IP sample to a new 1.5 ml tube with 150μl ChIP elution buffer and set aside until the IP’s are complete (these are the INPUT samples) 5.Add Ab to each IP sample except for the no-Ab control,nutate in cold room for 3 hrs 6.When there is 30 min
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