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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃,避免反复冻融
- 英文名:
T7 RNA Polymerase
- 库存:
99
- 供应商:
联硕生物
- CAS号:
9014-24-8
- 规格:
5ku
纯度:BR,50u/ul
保存温度:-20℃,避免反复冻融
英文名称:T7 RNA Polymerase
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文献和实验Here is our T7 siRNA protocol. The main idea is to design primers that begin with GG so thattranscription by T7 polymerase is efficient.I) FIRST design oligos:Using sense coding sequence of any gene....(N1, N2, N22, N23, are numbered positions
Cesium Chloride Purification of T7
Summary Cleaner stocks of T7 that concentrates and purifies T7 bacteriophage. Protocol Grow 100mL of permissive cells to a density of 108 to 109 cells/ml at 30°C in a rotary shaking water bath. Inoculate the cells
mRNA Amplification with T7 RNA Polymerase
on a 42ºC oven and set PCR machine to hold at 70ºC. In an Eppendorf tube add: 1 µg Total RNA 1 µL T7 oligo(dT) primer q.s. to 12 µL with Nuclease-free H2 O Incubate 10 min. at 70ºC. Spin briefly to pull
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