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- 保存条件:
2-8℃
- 库存:
99
- 供应商:
联硕生物
- 规格:
1g
保存温度:2-8℃
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文献和实验Yeast Transformation inoculate 5-10 ml culture with single colony and grow ON measure OD600nm and dilute to OD600nm = 0.3 in 50 ml grow for 2-4 hrs spin, wash cells with 25 ml H2 O spin
Using RNA Interference to Downregulate G Protein-Coupled Receptors
Technologies developed to interfere with gene transcripts were developed back in the 1980. However, it was not before the last decade that light was shed on the underlying mechanisms of what is now known as RNA interference
Yeast Chromatin Immunoprecipitation (ChIP)
Yeast Chromatin Immunoprecipitation (ChIP)Hahn LabJuly 2003Chromatin IP MethodDay 11.Start 5 ml o/n cultures of strains to be testedDay 21.Inoculate 50 ml of the desired media with a volume of a saturated o/n culture and grow o/n with shaking at 200
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