Menadione Sodium Bisulfite Hyd

Bisulfite Treatment of DNA

by adding 55 mg of hydroquinone to 50 ml of water. Add 520 ml freshly prepared 3M Sodium bisulfite (Sigma S-8890), prepared by adding 1.88 gm of sodium bisulfite per 5 ml of H2O, and adjusting pH to 5.0 with NaOH. Assure that reagents are mixed with DNA

Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Ampli

noindent Sodium bisulfite modification-based fine mapping of methylated cytosines represents the gold standard technique for DNA methylation studies. A major problem with this approach, however, is that it results in considerable DNA degradation

Bisulfite sequencing of very small samples

, remove NaOH solution. (to solidify agarose beads) In the meantime start preparing fresh 10 mM hydroquinone and 40.5% Sodium bisulfite in pure water (note 6). Both solutions are light sensitive, so try to avoid any unnecessary exposure to light