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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
Moracin M
- 库存:
99
- 供应商:
联硕生物
- CAS号:
56317-21-6
- 规格:
5mg
纯度:HPLC≥95%
保存温度:2-8℃
英文名称:Moracin M
分子式:C14H10O4
UKWTZBCQHDEYFPARVGIMSOXJLN
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文献和实验Single tube confirmation PCR protocol
concentration of ~10 µM). We use 5 µl of each primer in 50 µl PCR reactions (~ 1 µM final primer concentration). - Label 20 thin-wall PCR tubes (5 for each isolate) and add the following primer pairs: A-B, A-kanB, C-D, kanC-D, and A-D. Tubes 1-5
Clonality - X Chromosome Inactivation Assay
can be utilized. 1. Materials DNA sample (see Processing of Microdissected Tissue - DNA-based Analysis) Proteinase K (Sigma) Proteinase K buffer (0.05 M Tris-HCL, 0.001 M EDTA, 1% Tween 20, 0.1 mg/ml proteinase K, pH 8.0) Phenol:chloroform:isoamyl alcohol
T-cell dependent antigen specific in situ staining (Pe)
Double staining for Ag and Ki-67, TUNEL or BCL-6. Cut frozen tissue sections (spleen, lymph nodes) in a cryostat at ~ 4µm, on plain glass slides (Adhesive -coated slides are sometimes too hydrophobic, but fine
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