- ￥328.32
- AMEKO
- 中国
- AB66549-100mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃，避光
- 英文名：
Quinoline, 1,2,3,4-tetrahydro-3-me
- 库存：
99
- 供应商：
联硕生物
- CAS号：
20668-20-6
- 规格：
100mg
纯度：98%
保存温度：2-8℃，避光
英文名称：Quinoline, 1,2,3,4-tetrahydro-3-me
分子式：C10H13N
MRLNCTJPOZYXEGSQWFAIBVKHUD
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文献和实验Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) can be added in conjunction with NBT to increase the reaction rate further. Prepare a 100X stock of 2 mg/ml in H2O and use immediately. PMS is very unstable. To aid in identification of stained cells, the tissue can be processed for cryostat sections
. Test each tube w/H2O and discard.Add protein mixture to dialysis tube. Stir slowly in 100 mM Tris buffer or Binding buffer until pink color is out.Transfer protein mixture (Ab) to 50 ml Falcon tubes to determine volume.Dilute the mixture
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