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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 库存:
99
- 供应商:
联硕生物
- 规格:
250g
保存温度:RT
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文献和实验HOZAN-0641 镊子P-612-S 镊子 P-612-S HOZAN-0819 HOZAN宝山P-610-G镊子 P-610-G HOZAN-0088 镊子头P-815H-1 镊子头P-815H-1 HOZAN-0638 镊子P-620-S 镊子 P-620-S HOZAN-0640 镊子P-610-S 镊子 P-610-S HOZAN-0733 镊子头P-612S-1 P-612S-1 HOZAN-0081 耐热镊子P-815-H 耐热镊子P-815-H
Protein Expression and Purification of RPA70-AB (181-422)
.Transfer 200 µl of cells into 25 ml of LB containing required amount of antibiotics and set it in the 37 ℃ shaker at 200 rpm,O/N.3.Transfer the cells into 1 L LB containing antibiotics and incubate in the shaker at 250 rpm,at 37 ℃.4.Grow cells
Yeast Chromatin Immunoprecipitation (ChIP)
(these are the INPUT samples) 5. Add Ab to each IP sample except for the no-Ab control, nutate in cold room for 3 hrs 6. When there is 30 min left from step 5 prepare rProtein A (or G) Sepharose beads &Mac183; Spin down beads and remove supernatant &Mac183
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