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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
S-Bioallethrin
- 库存:
99
- 供应商:
联硕生物
- CAS号:
28434-00-6
- 规格:
10mg
纯度:98%
保存温度:-20℃
英文名称:S-Bioallethrin
分子式:C19H26O3
VBMEJKIQTZGANLUYOSWFDHXPCR
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文献和实验ELISA (Enzyme-Linked ImmunoadSorbent Assa
-conjugated R abbit A nti-M ouse) for Ab raised in mice and PSAR (P eroxidase-conjugated S wine A nti R abbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
-conjugated R abbit A nti-M ouse) for Ab raised in mice and PSAR (P eroxidase-conjugated S wine A nti R abbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins
Protocol for intracytoplasmic staining of cytokines for FACS analysis
. 2) Stimulate cells for 2 hours in vitro with 50 ng/ml PMA (Sigma, P8139) + 500 ng/ml ionomicin (Sigma I0634) in phenol red free RPMI 1640 medium + 10% fetal calf serum at 37 deg C. 3) Add Brefeldin A (Sigma, B7651) to 10mg/ml for a further 2 hr
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