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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
16-Epivoacarpine
- 库存:
99
- 供应商:
联硕生物
- CAS号:
114027-38-2
- 规格:
5mg
纯度:HPLC≥97%
保存温度:2-8℃
英文名称:16-Epivoacarpine
分子式:C21H24N2O4
NAFCTQOHVGBMUXYWRZLEKJSIDP
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文献和实验Phycoerythrin conjugation protocol
/ml (=0.7 ml) in PBS Add 16 µl SPDP (1.3 mg/ml in MeOH) Incubate at room temperature for 2.5 hours *** You must time steps 3 and 4 to finish at the same time, since the reaction products are unstable ***
T-cell dependent antigen specific in situ staining (Pe)
endogenous peroxidase by incubating in TBS-T 0.1%NaN 3 and 0.3% H 2 O 2 for 30 min. Wash thrice. Top of Page Choose next steps: HRP-conjugated secondary Ab Tyramide amplification Biotin-conjugated secondary Ab
Multiple Color Immunofluorescence for Cytokine Detection at the Single Cell Level
Upon proper stimulation (e.g., crosslinking FcγRIIIA [CD16], interaction with antibody [Ab]-nonsensitized target cells, cytokines), mature natural killer (NK) cells produce and release cytokines involved in the modulation of immune responses
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