- ￥1915.20
- AMEKO
- 中国
- AB61060-5mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 英文名：
Kizuta saponin K11
- 库存：
99
- 供应商：
联硕生物
- CAS号：
97240-03-4
- 规格：
5mg
纯度：HPLC≥98%
保存温度：2-8℃
英文名称：Kizuta saponin K11
分子式：C61H98O27
BWRQIFXDVOHYPTAEMCJKNSZLUG
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文献和实验Immunohistochemistry: Fluorescence Protocol 2.0
cytokine-specific antibodies (0.5-5 µg/mL) diluted in Wash Buffer-Saponin supplemented with 5% human AB serum. Wash the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room
Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
dish (can store for a year). Coat with polyamino acid. Coat coverslips in bulk in 10-15ml 1mg/ml PLL (or 500ug/ml polyornithine), rocking or rotating for a minimum of 30 minutes in a 10 or 15cm tissue culture dish. Save the polyamino acid
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