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- 中国
- AB60664-5mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 英文名：
1'-O-methyl neochebulinate
- 库存：
99
- 供应商：
联硕生物
- CAS号：
1236310-34-1
- 规格：
5mg
纯度：HPLC≥98%
保存温度：2-8℃
英文名称：1'-O-methyl neochebulinate
分子式：C42H36O28
VUXYWCMOKGLENBFPTSRADJIZQH
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文献和实验Optimizing Splice-Switching Oligomer Sequences Using 2-O-Methyl Phosphorothioate Chemistry
and then a set of oligomers complementary to the acceptor and donor splice sites, as well as intra-exonic regions predicted to contain exon splice enhancers, are designed and synthesized as 2′-O-methyl-modified bases on a phosphorothioate backbone (2OMeAOs
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST
down in Sorvall--5 K, 5 minutes, 4℃. Drain. Resuspend cells in 5 mL HEPES/sorb to wash. Spin down (5 K, S minutes, 4℃). Drain. Resuspend in 5 mL HEPES/sorb with 0.5 mM PMSF. Add 30-40 mL 1 mg/mL oxalyticase (can also use 2 mg zymolyase here, as preferred
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
(yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100ul/well) in 1x PBS
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