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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
9,10-Dioxo-9,10-dihydrophenanthrene-3-carboxylic acid
- 库存:
99
- 供应商:
鹿森生物
- CAS号:
32155-34-3
- 规格:
100mg
纯度:97%
保存温度:RT
英文名称:9,10-Dioxo-9,10-dihydrophenanthrene-3-carboxylic acid
分子式:C15H8O4
FIVTZQNWXOKEDSRAJMYPUCLHGB
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文献和实验Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
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