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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
Otophylloside T
- 库存:
99
- 供应商:
鹿森生物
- CAS号:
1642306-14-6
- 规格:
5mg
纯度:HPLC≥98%
保存温度:2-8℃
英文名称:Otophylloside T
分子式:C48H70O18
GDNBERLWJOTVPAHUFQYIMKCZSX
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文献和实验Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
T-cell dependent antigen specific in situ staining (Pe)
endogenous peroxidase by incubating in TBS-T 0.1%NaN 3 and 0.3% H 2 O 2 for 30 min. Wash thrice. Top of Page Choose next steps: HRP-conjugated secondary Ab Tyramide amplification Biotin-conjugated secondary Ab
Single and Double FISH protocols for Drosophila
, QC, Canada; Cat No. 1426 320). 5. Sheep anti-digoxigenin antibody (IgG; 1/1000 dilution of a 0.2 mg/ml stock solution; Boehringer Mannheim, Laval, QC, Canada; Cat No. 1333 089). 6. Goat anti-mouse antibody conjugated to CY2 [F(ab')2 fragment
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