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- 文献和实验
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- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10ml/20ml
| 规格: | 10ml | 产品价格: | ¥950.0 |
|---|---|---|---|
| 规格: | 20ml | 产品价格: | ¥1500.0 |
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文献和实验Single Quantum Dot Tracking of Membrane Receptors
fluorophores and fluorescent proteins rapidly photobleach, whereas gold particles and latex beads, although more stable, are bulky and usually stain only one protein per experiment. In comparison, QDs are bright and photostable fluorescent probes with a size
Sequenase[TM] catalyzed sequencing with dye-labeled terminators
to room temperature, alpha-thio-deoxynucleotides, fluorescent-labeled dye-terminators, and diluted Sequenase[TM] DNA polymerase are added and the mixture is incubated at 37degC. The reaction is stopped by adding ammonium acetate and ethanol, and the DNA
in each well is suctioned out with a mouth pipette.Each base-specific sequencing reaction terminated with the short termination mix is loaded using a mouth pipette onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA
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