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- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10ml/20ml
| 规格: | 10ml | 产品价格: | ¥950.0 |
|---|---|---|---|
| 规格: | 20ml | 产品价格: | ¥1500.0 |
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文献和实验DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL
: TBS 250 μl/well; 3x 30 seconds.8. Amplify signal by adding 100 μl/well AttoPhos Fluorescent substrate system, for 5-10 min at RT. 36 mg ofAttoPhos substrate should be mixed with 60 ml of AttoPhos buffer 24 hours prior to use. Make sure well isclean
BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
: For most cell lines tested (e.g. HEK293, A549, HeLa), we obtain a readily detectable fluorescence signal when using 100 nM BLOCK-iT™ Fluorescent Oligo for transfection. Prepare and seed mammalian cells at a density recommended by the manufacturer
FLUORESCENT STAINING OF CELLS Fluorescent Phalloidin Staining Materials 1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2. PBS
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