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文献和实验Phycoerythrin conjugation protocol
reagents and incubate at 4 °C overnight on rotary mixer. Add 80 µl N-ethyl maleimide (0.1 mM) to reaction mixture to stop reaction. Agitate and incubate 30 mins at room temperature Concentrate to 0.5 ml or less on Centricon
Conjugation of Antibody to HRP
:EDTA Add 0.25 g NaOH pellets to this solution to neutralize to approximately pH 7.0 Add to antibody solution at 6.49 ml/ml SATA. Gently stir for 30 minutes at room temperature. Equilibrate column with PBS:EDTA. Desalt thiolated antibody
RAT/MOUSE GROWTH HORMONE ELISA KIT
polyclonal antibodies, 2) wash away of unbound materials from samples, 3) binding of a second biotinylated anti-Growth Hormone polyclonal antibody to the captured molecules, 4) wash away of unbound materials from samples, 5) conjugation of horseradish
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