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SIGMA P7936-10MG 磷酸肌酸二钠盐 19333

-65-4
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  • ¥320
  • Sigma-Aldrich
  • 进口
  • P7936-10MG
  • 2025年08月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      −20°C

    • 保质期

      根据瓶身LOT号查询

    • 英文名

      Phosphocreatine disodium salt hydrate

    • 库存

      有现货

    • 供应商

      浙江羽翔生物科技有限公司

    • CAS号

      19333-65-4

    • 规格

      10MG

    属性

    Product Name

    磷酸肌酸 二钠盐 水合物, ≥97%

    质量水平

    200

    方案

    ≥97%

    表单

    powder

    杂质

    ≤0.02% free creatine

    颜色

    white

    溶解性

    water: 50 mg/mL, clear, colorless

    储存温度

    −20°C

    SMILES字符串

    O.[Na+].[Na+].CN(CC(O)=O)C(=N)NP([O-])([O-])=O

    InChI

    1S/C4H10N3O5P.2Na.H2O/c1-7(2-3(8)9)4(5)6-13(10,11)12;;;/h2H2,1H3,(H,8,9)(H4,5,6,10,11,12);;;1H2/q;2*+1;/p-2

    InChI key

    KZVYPOKBYRXZKS-UHFFFAOYSA-L

    应用

    磷酸肌酸二钠盐水合物已用于:
    • 作为爪蟾缓冲液(XB)组分,用于去除膜吸附性蛋白质
    • 作为M5+缓冲液组分,进行极化全内反射荧光(polTIRF)实验
    • 作为ATP再生系统组分,用于7-硝基-2-1,3-苯并噁二唑-4-基磷脂(NBD-PL)转位检测

    生化/生理作用

    磷酸肌酸(Phosphocreatine)因其在肌酸激酶作用下的最快再生形式而广为人知,起到临时能量缓冲器的作用。磷酸肌酸存在于肌肉组织中,在维持肌肉细胞的能量稳态中起重要作用。
    磷酸肌酸二钠盐水合物可用于研究鼻咽癌细胞和诱发的暂时性心脏骤停。它也用于评估伏隔核中腺苷A(2A)受体调节对大鼠可卡yin寻求行为的影响。
    磷酸肌酸是脊椎动物和某些无脊椎动物肌肉的高能磷酸基储蓄池,提供ADP-ATP转换所需磷酸。

    制备说明

    酶法制备。

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    图标文献和实验
    该产品被引用文献

    Role of the phosphocreatine system on energetic homeostasis in skeletal and cardiac muscles.

    Einstein (Sao Paulo, Brazil) (2014-04-15)
    Lucas Guimarães-Ferreira
    PMID24728259
    摘要

    Adenosine triphosphate is the present energy currency in the body, and is used in various cellular and indispensable processes for the maintenance of cell homeostasis. The regeneration mechanisms of adenosine triphosphate, from the product of its hydrolysis - adenosine diphosphate - are therefore necessary. Phosphocreatine is known as its quickest form of regeneration, by means of the enzyme creatine kinase. Thus, the primary function of this system is to act as a temporal energy buffer. Nevertheless, over the years, several other functions were attributed to phosphocreatine. This occurs as various isoforms of creatine kinase isoforms have been identified with a distinct subcellular location and functionally coupled with the sites that generate and use energy, in the mitochondria and cytosol, respectively. The present study discussed the central and complex role that the phosphocreatine system performs in energy homeostasis in muscle cells, as well as its alterations in pathological conditions.

    相关实验
    • Analysis of Genomic DNA by Southern Hybridization

      . 0.05M Tris・Cl pH8.0, 0.5mM EDTA, 1% SDS, and 10% Dextran). Boil 40µl (10mg/ml) cold nonspecific DNA (Sigma, human placental DNA) in 400µl TE (pH8.0) for 5 min. and add to the hybridization buffer. Hybridize the filter with cold nonspecific

    • Metabolic labeling Immunoprecipitation

      /ml Cysteine-HCl,100µg/ml methionine,sterile filtered • Tris-buffered saline (TBS): 20mM Tris,150mM NaCl,pH 7.4 • X1000 Protease inhibitor soln: 500mM PMSF (Sigma P-7626)in EtOH,10mg/ml leupeptin (Sigma L-2023)in water,10mg/ml pepstatin A (P-4265

    • Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

      (Boehringer Mannheim Cat# 242 608)- Levamisole (Sigma, Cat# L-9756) - NaN3 (Sigma Cat# S-2002)- NBT: 4-Nitro blue tetrazolium chloride. (Boehringer Mannheim Cat# 1383 213)- p-Phenylenediamine (Sigma Cat# P-6001)- 0.1% Poly-L-lysine solution

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    SIGMA P7936-10MG 磷酸肌酸二钠盐 19333-65-4
    ¥320