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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
−20°C
- 保质期:
根据瓶身LOT号查询
- 英文名:
Phosphocreatine disodium salt hydrate
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
19333-65-4
- 规格:
10MG
属性
Product Name
磷酸肌酸 二钠盐 水合物, ≥97%
质量水平
200
方案
≥97%
表单
powder
杂质
≤0.02% free creatine
颜色
white
溶解性
water: 50 mg/mL, clear, colorless
储存温度
−20°C
SMILES字符串
O.[Na+].[Na+].CN(CC(O)=O)C(=N)NP([O-])([O-])=O
InChI
1S/C4H10N3O5P.2Na.H2O/c1-7(2-3(8)9)4(5)6-13(10,11)12;;;/h2H2,1H3,(H,8,9)(H4,5,6,10,11,12);;;1H2/q;2*+1;/p-2
InChI key
KZVYPOKBYRXZKS-UHFFFAOYSA-L
应用
- 作为爪蟾缓冲液(XB)组分,用于去除膜吸附性蛋白质
- 作为M5+缓冲液组分,进行极化全内反射荧光(polTIRF)实验
- 作为ATP再生系统组分,用于7-硝基-2-1,3-苯并噁二唑-4-基磷脂(NBD-PL)转位检测
生化/生理作用
制备说明
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文献和实验Role of the phosphocreatine system on energetic homeostasis in skeletal and cardiac muscles.
Adenosine triphosphate is the present energy currency in the body, and is used in various cellular and indispensable processes for the maintenance of cell homeostasis. The regeneration mechanisms of adenosine triphosphate, from the product of its hydrolysis - adenosine diphosphate - are therefore necessary. Phosphocreatine is known as its quickest form of regeneration, by means of the enzyme creatine kinase. Thus, the primary function of this system is to act as a temporal energy buffer. Nevertheless, over the years, several other functions were attributed to phosphocreatine. This occurs as various isoforms of creatine kinase isoforms have been identified with a distinct subcellular location and functionally coupled with the sites that generate and use energy, in the mitochondria and cytosol, respectively. The present study discussed the central and complex role that the phosphocreatine system performs in energy homeostasis in muscle cells, as well as its alterations in pathological conditions.
Analysis of Genomic DNA by Southern Hybridization
. 0.05M Tris・Cl pH8.0, 0.5mM EDTA, 1% SDS, and 10% Dextran). Boil 40µl (10mg/ml) cold nonspecific DNA (Sigma, human placental DNA) in 400µl TE (pH8.0) for 5 min. and add to the hybridization buffer. Hybridize the filter with cold nonspecific
Metabolic labeling Immunoprecipitation
/ml Cysteine-HCl,100µg/ml methionine,sterile filtered • Tris-buffered saline (TBS): 20mM Tris,150mM NaCl,pH 7.4 • X1000 Protease inhibitor soln: 500mM PMSF (Sigma P-7626)in EtOH,10mg/ml leupeptin (Sigma L-2023)in water,10mg/ml pepstatin A (P-4265
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
(Boehringer Mannheim Cat# 242 608)- Levamisole (Sigma, Cat# L-9756) - NaN3 (Sigma Cat# S-2002)- NBT: 4-Nitro blue tetrazolium chloride. (Boehringer Mannheim Cat# 1383 213)- p-Phenylenediamine (Sigma Cat# P-6001)- 0.1% Poly-L-lysine solution
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