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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide of ATPB in Oryza sativa Os05g0553000
- 亚型:
IgG
- 保存条件:
Store at -20℃. Avoid freeze/thaw cycles.
- 克隆性:
Polyclonal Antibody
- 标记物:
Biotin,Cy3,Cy3.5,Cy5,Cy5.5,Cy7,FITC,HRP,RBITC,Solar Fluor 350,Solar Fluor 405,Solar Fluor 488,Solar Fluor 532,Solar Fluor 555,Solar Fluor 568,Solar Fluor 594,Solar Fluor 633,Solar Fluor 640,Solar Fluor 647,Solar Fluor 660,Solar Fluor 680,Solar Fluor 750
- 适应物种:
Triticum aestivum;Nicotiana tabacum;Arabidopsis thaliana;Oryza sativa;Zea mays;Glycine max
- 抗原来源:
KLH-conjugated synthetic peptide of ATPB in Oryza sativa Os05g0553000
- 目录编号:
4339546
- 级别:
实验级别
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
WB 1:5000-8000.
- 靶点:
ATPB
- 抗体英文名:
Anti-ATPB polyclonal Antibody for Plant
- 抗体名:
ATP synthase subunit beta, mitochondrial
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2350.0 |
| 基本信息 | |
| 英文名称 | Anti-ATPB polyclonal Antibody for Plant |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Triticum aestivum;Nicotiana tabacum;Arabidopsis thaliana;Oryza sativa;Zea mays;Glycine max |
| 预测交叉反应 | Hevea brasiliensis |
| 应用 | WB |
| 稀释比例 | WB 1:5000-8000. |
| 密度 | |
| 靶标 | ATPB |
| 别名 | ATP synthase subunit beta, mitochondrial |
| 背景说明 | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
| Swiss Prot | Q01859 |
| Gene ID | 4339546 |
| 亚细胞定位 | Mitochondrion |
| 免疫原 | KLH-conjugated synthetic peptide of ATPB in Oryza sativa Os05g0553000 |
| 理论分子量 | 59 kDa |
| 实际分子量 | 59 kDa |
| EC | |
| 纯化方法 | Protein A |
| 储存液 | PBS with 50% Glycerol,0.03% Proclin300,0.5% BSA,pH 7.3. |
| 储存条件 | Store at -20℃. Avoid freeze/thaw cycles. |
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文献和实验Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions
we describe a method to generate plant protein microarrays and to use them for characterizing monoclonal antibodies or polyclonal sera with regard to their specificity and cross-reactivity. The method starts with characterized E. coli cDNA expression clones encoding
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
suspended in PBS. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600
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