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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
PROGOITRIN POTASSIUM SALT(P)
- 库存:
99
- 供应商:
鹿森生物
- CAS号:
21087-77-4
- 规格:
1mg
纯度:HPLC≥98%
保存温度:-20℃
英文名称:PROGOITRIN POTASSIUM SALT(P)
分子式:C11H18NO10S2K
OPESNYCUBAXRFJQVDLKZHMWTIG
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文献和实验Expression L19 using Pichia pastoris
Strike out some glycerol on a YPD+0.1mg/ml Z agar plate. Pick up one colony and start to grow the culture. 1. Growing culture Inoculate a clone in a sterile 2l flask containing 600 ml YPD + 0.1 mg/ml Zeocine. Use anti-foam agent if necessary
Site-directed hydroxy radical mapping of nucleosome positions in vitro
12). Mapping Reaction A typical sample for mapping has a total volume of 10-20µl and contains 0.2-0.5µM reconstituted nucleosome, 50mM Tris-Cl pH 7.5 and appropriate monovalent salt. After desired experimental manipulation, place sample
的一块,4度离心后又多了白色沉定,SDS结晶?测浓度,含量才1mg/ml左右。解决:提蛋白试剂盒,另外组织大小适中,要碎,立即加2X BUFFER,然后煮5-10分钟,效果很好的。四、lysis solution:(yog)Protein extraction buffer (Camiolo buffer):100 ml= (0.075M Potassium Acetate) 0.736g(0.3M) NaCl 1.753g(0.1M) L-arginine basic salt 1.742g
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