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- Millipore
- LSKMAGH02
- 2025年08月12日
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文献和实验. Lineage Depletion: 1. Resuspend at a density of 5x107 cells/mL in PBS/FBS plus antibody cocktail (each antibody final dilution = 1/500 v/v). 2. Incubate on ice for 30 min. Meanwhile, wash Dynabeads 2x in PBS/FBS. Resuspend the beads
Affinity chromatography (with HIS-tagged proteins)
in artifactual interactions. The poly-his tag binds to a nickel chelate resin for creation of the column. We use the resin alone for the control columns. Most often, we challenge our columns with 35S-labeled, in vitro translated proteins, however, proteins
digestion See SAGE protocol Step 3. Bind Biotinylated cDNA to magnetic beads See SAGE protocol. Step 4. Linker ligation See SAGE protocol and below. Only linker 2A and 2B are used in rSAGE. They are used at one fifth of that used in SAGE
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