Immobilon® -NC 硝酸纤维素转印膜，预切片膜，0

Mag-Bind® mRNA Protocol (Standard Protocol)

by adding 750 ul of isopropanol per 1.5 mL of RNA-Solv ® Reagent used for the initial homogenization. Invert the sample 10-20 times. Incubate samples at room temperature 10 minutes and centrifuge at no more than 12,000 x g for 10 minutes at 2-8°C.

Separation of Histone Variants and Post‐Translationally Modified Isoforms by Triton/Acetic Acid/Urea Polyacrylamide Gel Electrophoresis

of gels containing the nonionic detergent Triton X?100, referred to as Triton/acetic acid/urea (TAU) polyacrylamide gels, for analysis of histones. Also included are support protocols detailing several accessory techniques: assembly of gel plates

发一个invitrogen公司网站上的“Western转膜步骤”，其中有些观点不错

是该公司结合自己产品的一个说明，但其中有些知识我自己认为很有用，转下来。 下面的步骤适用于通过Xcell Ⅱ转印系统进行蛋白印记的大部分应用。为达到最佳效果，用户的优化是必要的。 I. 所需材料： • Xcell SureLock™或Xcell Ⅱ™ Mini-Cell以及Blot Module（Cat. Nos. EI10001, EI9001及EI9051） • 电泳后的迷你胶（mini-gel）（最大的尺寸9cm*9cm） • 预先切割好的印记膜： 硝酸纤维素（Cat