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- Millipore
- ISEQ15150
- 2025年08月12日
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文献和实验the membrane 3 x 10 min 3 x 5 min Incubate with second antibody 1 hr 30 min Wash the membrane 3 x 10 min 3 x 5 min Add substrate 5 min
. The three-dimensional structure of PrPC is characterized by a high ?-helical content (9 ); all or part of it undergoes a posttranslational modification to β-sheet in PrPSc (10 -12 ). Although PrPC (33-35 kDa) is completely hydrolyzed by protease treatment, PrPSc
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37?C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells by centrifugation for 20’ at 3,200 RPM in a Sorvall RT7 at 4?C with RTH-250 rotor, ~1700 xg. Decant and drain pellets
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