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- 详细信息
- 文献和实验
- 技术资料
- 库存:
充足
- 英文名:
10 × BsaI Digestion Buffer (GMP Grade)
- 供应商:
南京诺唯赞生物科技股份有限公司
- 保存条件:
见详情页
- 规格:
5 ml/25 ml/100 ml
产品描述
10 × BsaI Digestion Buffer 是专为以BsaI (20 U/μl, GMP Grade)(Vazyme #GMP4303PC)进行酶切DNA优化的反应缓冲液。
储存条件
-30 ~ -15℃密封保存,有效期24个月。
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文献和实验In-gel digestion of proteins for peptide fingerprint mapping
stained polyacrylamide gels. First, SDS is removed from the gel prior to digestion, in several ammoniumcarbonate/acetonitrile washing steps. The excised gel pieces are subsequently dried and rehydrated with enzyme in buffer. Alternatively, the washed gel
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
) and other (buffer) contaminants (which may give a higher background during MS measurement) Wash the sample 5 min in 80 µl 0.1M NH4HCO3 Add an equal volume of 100% Acetonitrile and vortex for 5 min Spin down the gel particles (table-top centrifuge
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
with the peptides) and other (buffer) contaminants (which may give a higher background during MS measurement) Wash the sample 5 min in 80 µl 0.1M NH4HCO3 Add an equal volume of 100% Acetonitrile and vortex for 5 min Spin down the gel
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