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- 禾午生物
- P69310
- 国产
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pT7-12×His-LEP(human)-MT4(human-opt-ecoli)
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pT7-12×His-LEP(human)-MT4(human-opt-ecoli) |
| 别称 | NM_000230;LEPD; OB; OBS;NM_032935.3;MT-4; MT-IV; MTIV |
| 基因长 | 501bp+186bp |
| 质粒宿主 | 大肠杆菌 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 人 |
| 原核抗性 | Kan |
| 启动子 | T7 |
| 复制子 | pBR322 |
| 感受态 | DH5a |
| 温度 | 37度 |
| 背景载体 | pET-28a |
| 正向引物 | T7 |
| 拷贝数 | 低 |
| pT7-6×His-TEV-GTF2H1(human-opt-human) |
| pT7-6×His-EGFP-TFAM(human) |
| pT7-6×His-NLRP3(human) |
| pTac-GST-OTUD4(human) |
| pT7-6×His-NCL(human)-6×His |
| pT7-6×His-mCherry-DDX5(human) |
| pT7-TrxA-6×His-ELOA(human)-6×His |
| pT7-6×His-SUMO-TEV-GTF2H1(human-opt-human) |
| pTac-GST-EGFP-NPSR1(human) |
| pT7-6×His-UBE3A(human)-6×His |
| pT7-6×His-EPHB4(human)-6×His |
| pTac-GST-TEV-GTF2H1(human-opt-human) |
| pT7-6×His-EGFP-ntt1(Bacteria)-6×His |
| pT7-6×His-EGFP-NANOG(human) |
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文献和实验Retroviral infection of primary human keratinocytes (PHKs)
Packaging cells grow very fast especially PT67s (12-hour doubling time) Overconfluence is not so bad (titres remain OK) Half-life of virus is about 4 hours in media at 37ºC. Supernatants are useful at anything from 6 hours to 24 hours
Human Peripheral Blood Mononuclear Cell Preparation
-Hypaque in four 50 ml conical tubes (15 ml Ficoll-Hypaque per tube).8. Centrifuge at 800×g for 20 min at 12℃ with the BRAKE OFF.9. The mononuclear leukocytes should appear as a cloudy ring at the PBS/Ficoll interface. Harvest them from the interface
PriCells: Methods of Isolation and Culture of Human Hepatocytes
should be viewed using a standard laboratory 16 binocular microscope (×400 magnification). Typically 70–90% of isolated human hepatocytes exclude trypan blue. Suspensions of isolated human hepatocytes can be used for short-term experiments, i.e., up to 4 h
技术资料