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- BC151139.1(1点缺失);ZSCAN31
- 上海
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 提供商：
上海博尔森生物科技有限公司
- 服务名称：
pCR-XL-TOPO-ZKSCAN2(human)(1点缺失)
- 规格：
干粉质粒
pCR-XL-TOPO-ZKSCAN2(human)(1点缺失)
[ 产品信息 ]
[ 质粒简介 ]
pCR-XL-TOPO-ZKSCAN2(human)(1点缺失)
[ 参考序列 ]
CTTACCTACATAGGGCGATTGGGCCCTCTAGATGCATGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTCTTTGGGTCCCCACCTTGAGCTAGACGTGAAGTTGACCCCTGGGCTGCAGCATGGCTGTCGCCCTCGACTCTCAGATCGACGCGCCCCTGGAGGTTGAGGGATGCCTAATAATGAAGGTGGAAAAGGACCCCGAGTGGGCATCAGAGCCCATTCTGGAAGGATCGGATAGCTCTGAGACCTTCCGCAAATGCTTCAGGCAATTCTGTTATGAGGATGTGACTGGACCCCATGAAGCTTTCAGTAAACTCTGGGAACTTTGCTGCCGGTGGCTGAAGCCAGAAATGCGTTCCAAGGAGCAAATACTTGAGCTGCTGGTGATTGAGCAGTTTCTCACCATTTTACCCGAGAAGATTCAGGCTTGGGCACAGAAGCAGTGTCCGCAAAGTGGAGAGGAAGCGGTGGCCCTGGTAGTGCATTTGGAGAAAGAGACTGGAAGACTAAGACAGCAGGTCAGCAGTCCCGTGCACCGGGAGAAGCACTCCCCACTTGGAGCAGCGTGGGAGGTGGCAGACTTCCAGCCAGAGCAGGTGGAGACCCAACCCAGGGCGGTGTCTCGGGAGGAACCTGGAAGCCTCCACTCAGGACACCAGGAACAGCTGAACCGAAAGCGAGAACGTCGGCCCTTACCCAAGAATGCTCGGCCTTCTCCCTGGGTTCCTGCCCTTGCTGATGAATGGAATACCCTAGATCAGGAAGTGACAACCACACGGCTTCCTGCTGGGTCCCAGGAACCAGTGAAAGATGTCCACGTGGCCAGAGGCTTTTCCTACAGAAAGAGTGTGCATCAGATTCCTGCCCAAAGGGACCTCTACCGGGATTTCAGGAAGGAGAATGTTGGGAACGTGGTCTCCCTGGGAAGTGCAGTGTCTACATCTAACAAGATAACCCGGTTGGAACAGAGAAAGGAGCCATGGACTCTAGGTCTGCATTCCTCTAACAAGAGAAGTATCCTACGAAGCAACTACGTCAAGGAAAAGTCAGTTCATGCTATTCAGGTCCCTGCAAGGAGTGCAGGAAAAACATGGAGAGAGCAGCAGCAGTGGGGTTTAGAAGATGAAAAGATAGCAGGTGTGCATTGGAGCTATGAGGAAACAAAGACTTTCCTGGCAATTCTCAAAGAGTCTCGCTTTTATGAAACACTTCAGGCCTGTCCCCGAAATAGCCAAGTGTATGGTGCTGTGGCTGAATGGTTGCGAGAATGTGGCTTCCTTAGAACCCCAGAACAGTGTCGAACCAAGTTCAAAAGTCTCCAGAAAAGCTATCGAAAGGTGAGAAATGGCCACATGCTAGAACCCTGCGCCTTCTTTGAGGACATGGATGCTTTGTTGAACCCTGCAGCCCGTGCTCCGTCCACTGATAAACCAAAGGAGATGATACCTGTCCCCAGACTGAAGAGAATTGCCATCAGTGCTAAGGAACACATCAGCTTGGTGGAGGAGGAGGAAGCTGCAGAAGATTCTGATGATGATGAAATAGGCATCGAATTTATCCGCAAGTCTGAAATCCATGGTGCCCCTGTCTTGTTTCAGAATCTCAGTGGCGTGCACTGGGGCTATGAAGAAACCAAGACTTTTCTTGATATCCTCCGTGAGACTCGGTTTTATGAAGCGCTTCAAGCCTGTCATCGGAAGAGCAAATTGTATGGGGCTGTAGCTGAACAGCTTCGAGAGTGCGGCTTCCTCCGGACACCAGAACAGTGCCGAACCAAGTTCAAAAGCCTTCAGAAGAGTTACCGCAAGGTGAAAAATGGCCACGTGCTAGAGTCCTGCGCGTTCTACAGGAGATGGATGCCCTGATTAACTCTCGGGCATCTGCTCCTTCCCCCAGCACCCCAGAGGAAGTCCCATCACCTTCAAGGCAAGAAAGAGGGGGTATTGAGGTTGAACCCCAGGATCCTACAGGCTGGGAACCTGAAGAGACCTCACAGGAGGCAGTAATAGAAGACTCTTGCAGTGAGAGAATGAGCGAGGAGGAAATTGTGCAAGAGCCAGAGTTCCAGGGACCTCCAGGTCTACTGCAGAGCCCAAATGATTTTGAAATCGGAAGTAGCATCAAGGAGGATCCAACACAGATAGTATATAAGGACATGGAACAGCATAGGGCATTAATAGAAAAGTCTAAAAGAGTTGTTTCCCAGAGTACCGACCCCAGCAAATATCGCAAAAGGGAATGCATCTCAGGAAGACAATGGGAAAATCTTCAAGGAATTAGACAGGGAAAGCCGATGTCTCAGCCTAGAGATTTAGGGAAAGCCGTTGTGCATCAGAGGCCTTTTGTGGGGAAGAGACCCTACAGACTTCTCAAATATGGAGAAAGCTTTGGAAGGAGCACTCGTCTGATGTGCCGGATGACCCACCACAAGGAGAATCCTTACAAGTGTGGTGTCTGTGGGAAGTGCTTTGGTAGAAGCAGGAGCCTGATCAGACACCAAAGAATCCACACAGGCGAAAAACCTTTTAAATGTCTTGACTGTGGAAAAAGCTTTAATGACTCCTCAAATTTTGGTGCCCACCAGAGAATCCACACAGGAGAGAAACCCTACAGATGCGGAGAGTGTGGAAAATGCTTTAGTCAGAGCTCTAGTCTTATTATACATCAGAGAACGCACACCGGTGAGAAGCCCTATCAGTGTGGAGAGTGTGGGAAAAGTTTCACCAACAGTTCTCATTTCAGCGCCCACCGGAGAGTTCACACTGGGGAGAATCCCTACAAATGTGTGGACTGTGAAAAAAGTTTCAATAACTGTACGAGATTTCGAGAACATCGGAGAATACACACTGGAGAGAAGCCCTATGGATGTGCCCAGTGTGGCAAACGTTTCAGTAAGAGTTCTGTTCTTACCAAACATCGGGAAGTTCATGTGAGAGAAAAGCCTCTGCCACACCCTCCATCTCTGTATTGCCCTGAGAACCCACATAAGGGAAAGACTGATGAATTTAGGAAAACTTTTTGATGATGATCTTCTCTTCCTAAATGCCCCCTTAGCCATTTTACCCTAATCTCATTCTTGGAAGCAATCTTTTCTTAGCTATGAAGTGTAATTTCCAAGATAAACTTGAGAATATAAATAAACTAAACACTTTCATCCTGTTCTCACCTCTGCCTCTAACTTCATCAGTCTGTTCCTCCTCTTCTGTGTGTCTCAGCTTCTCCTTTAAGAATGTGGCGAAGATACATTGTCCGAGTTCAGAATGAAATACCCTTCAGTACATTGAGGCCACTGCAACTGGATTGCTAAGTTCGGCAACATTCAAGATCTTCCATATGGGTGAGAGTTGTCTTCAAGGATATAAAAAATGCCAAAAGTTTTTTTGTTGTTATTACCTACTCCGTGAGATCCTGGATGAAACTTGATAACAGATTTTCACATTTAGATAGAACTTTGAGGAGTCTGATCGGAACTACATCAAATCCATCTTGGTTTTGAAGAGATCCACAAAAGAAGAGTGAAGATTTTATAGCTTTCCTCAGAAGTCTGTTACAGTGTACCTTTTTTTTTTTTTTGTCAAGAAGTTGATCCAAATAGCTAGGGCAGAATCCCTGTATTGTCACTGAAATCCTGTTACTAATGCCTTGCCCTCCATGGAGGTGAGAAGCAATTGATCACCCCTCATCCTTTTCTGTACCCGAAGACTTAAATCCCCTGGCTGCTGCTTCTCCAGCCTATAAGTAATCCCAGGTCCTCCTACTGGTTCTCATAGCCCTGGACTTGCCTCCCGATGGTCTGGGGGGTCCTGGCGGGCT
注意事项：该产品仅供科研实验使用，未经允许不得用于其它用途！以实际收货产品说明书为准，网站说明书仅供参考。
[ 产品信息 ]
| 名称 | pCR-XL-TOPO-ZKSCAN2(human)(1点缺失) | 别称 | BC151139.1(1点缺失);ZSCAN31 |
|---|---|---|---|
| 基因长 | 2904bp | 质粒宿主 | 大肠杆菌 |
| 质粒用途 | 基因模板 | 片段类型 | cDNA |
| 片段物种 | 人 | 原核抗性 | Kan |
| 货号: | BES226273PVE | 荧光标记 | |
| 启动子 | 复制子 | pUC | |
| 感受态 | DH5a | 温度 | 37度 |
| 背景载体 | 正向引物 | M13F：TGTAAAACGACGGCCAGT | |
| 反向引物 | M13R：CAGGAAACAGCTATGACC | 拷贝数 |
pCR-XL-TOPO-ZKSCAN2(human)(1点缺失)
[ 参考序列 ]
CTTACCTACATAGGGCGATTGGGCCCTCTAGATGCATGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTCTTTGGGTCCCCACCTTGAGCTAGACGTGAAGTTGACCCCTGGGCTGCAGCATGGCTGTCGCCCTCGACTCTCAGATCGACGCGCCCCTGGAGGTTGAGGGATGCCTAATAATGAAGGTGGAAAAGGACCCCGAGTGGGCATCAGAGCCCATTCTGGAAGGATCGGATAGCTCTGAGACCTTCCGCAAATGCTTCAGGCAATTCTGTTATGAGGATGTGACTGGACCCCATGAAGCTTTCAGTAAACTCTGGGAACTTTGCTGCCGGTGGCTGAAGCCAGAAATGCGTTCCAAGGAGCAAATACTTGAGCTGCTGGTGATTGAGCAGTTTCTCACCATTTTACCCGAGAAGATTCAGGCTTGGGCACAGAAGCAGTGTCCGCAAAGTGGAGAGGAAGCGGTGGCCCTGGTAGTGCATTTGGAGAAAGAGACTGGAAGACTAAGACAGCAGGTCAGCAGTCCCGTGCACCGGGAGAAGCACTCCCCACTTGGAGCAGCGTGGGAGGTGGCAGACTTCCAGCCAGAGCAGGTGGAGACCCAACCCAGGGCGGTGTCTCGGGAGGAACCTGGAAGCCTCCACTCAGGACACCAGGAACAGCTGAACCGAAAGCGAGAACGTCGGCCCTTACCCAAGAATGCTCGGCCTTCTCCCTGGGTTCCTGCCCTTGCTGATGAATGGAATACCCTAGATCAGGAAGTGACAACCACACGGCTTCCTGCTGGGTCCCAGGAACCAGTGAAAGATGTCCACGTGGCCAGAGGCTTTTCCTACAGAAAGAGTGTGCATCAGATTCCTGCCCAAAGGGACCTCTACCGGGATTTCAGGAAGGAGAATGTTGGGAACGTGGTCTCCCTGGGAAGTGCAGTGTCTACATCTAACAAGATAACCCGGTTGGAACAGAGAAAGGAGCCATGGACTCTAGGTCTGCATTCCTCTAACAAGAGAAGTATCCTACGAAGCAACTACGTCAAGGAAAAGTCAGTTCATGCTATTCAGGTCCCTGCAAGGAGTGCAGGAAAAACATGGAGAGAGCAGCAGCAGTGGGGTTTAGAAGATGAAAAGATAGCAGGTGTGCATTGGAGCTATGAGGAAACAAAGACTTTCCTGGCAATTCTCAAAGAGTCTCGCTTTTATGAAACACTTCAGGCCTGTCCCCGAAATAGCCAAGTGTATGGTGCTGTGGCTGAATGGTTGCGAGAATGTGGCTTCCTTAGAACCCCAGAACAGTGTCGAACCAAGTTCAAAAGTCTCCAGAAAAGCTATCGAAAGGTGAGAAATGGCCACATGCTAGAACCCTGCGCCTTCTTTGAGGACATGGATGCTTTGTTGAACCCTGCAGCCCGTGCTCCGTCCACTGATAAACCAAAGGAGATGATACCTGTCCCCAGACTGAAGAGAATTGCCATCAGTGCTAAGGAACACATCAGCTTGGTGGAGGAGGAGGAAGCTGCAGAAGATTCTGATGATGATGAAATAGGCATCGAATTTATCCGCAAGTCTGAAATCCATGGTGCCCCTGTCTTGTTTCAGAATCTCAGTGGCGTGCACTGGGGCTATGAAGAAACCAAGACTTTTCTTGATATCCTCCGTGAGACTCGGTTTTATGAAGCGCTTCAAGCCTGTCATCGGAAGAGCAAATTGTATGGGGCTGTAGCTGAACAGCTTCGAGAGTGCGGCTTCCTCCGGACACCAGAACAGTGCCGAACCAAGTTCAAAAGCCTTCAGAAGAGTTACCGCAAGGTGAAAAATGGCCACGTGCTAGAGTCCTGCGCGTTCTACAGGAGATGGATGCCCTGATTAACTCTCGGGCATCTGCTCCTTCCCCCAGCACCCCAGAGGAAGTCCCATCACCTTCAAGGCAAGAAAGAGGGGGTATTGAGGTTGAACCCCAGGATCCTACAGGCTGGGAACCTGAAGAGACCTCACAGGAGGCAGTAATAGAAGACTCTTGCAGTGAGAGAATGAGCGAGGAGGAAATTGTGCAAGAGCCAGAGTTCCAGGGACCTCCAGGTCTACTGCAGAGCCCAAATGATTTTGAAATCGGAAGTAGCATCAAGGAGGATCCAACACAGATAGTATATAAGGACATGGAACAGCATAGGGCATTAATAGAAAAGTCTAAAAGAGTTGTTTCCCAGAGTACCGACCCCAGCAAATATCGCAAAAGGGAATGCATCTCAGGAAGACAATGGGAAAATCTTCAAGGAATTAGACAGGGAAAGCCGATGTCTCAGCCTAGAGATTTAGGGAAAGCCGTTGTGCATCAGAGGCCTTTTGTGGGGAAGAGACCCTACAGACTTCTCAAATATGGAGAAAGCTTTGGAAGGAGCACTCGTCTGATGTGCCGGATGACCCACCACAAGGAGAATCCTTACAAGTGTGGTGTCTGTGGGAAGTGCTTTGGTAGAAGCAGGAGCCTGATCAGACACCAAAGAATCCACACAGGCGAAAAACCTTTTAAATGTCTTGACTGTGGAAAAAGCTTTAATGACTCCTCAAATTTTGGTGCCCACCAGAGAATCCACACAGGAGAGAAACCCTACAGATGCGGAGAGTGTGGAAAATGCTTTAGTCAGAGCTCTAGTCTTATTATACATCAGAGAACGCACACCGGTGAGAAGCCCTATCAGTGTGGAGAGTGTGGGAAAAGTTTCACCAACAGTTCTCATTTCAGCGCCCACCGGAGAGTTCACACTGGGGAGAATCCCTACAAATGTGTGGACTGTGAAAAAAGTTTCAATAACTGTACGAGATTTCGAGAACATCGGAGAATACACACTGGAGAGAAGCCCTATGGATGTGCCCAGTGTGGCAAACGTTTCAGTAAGAGTTCTGTTCTTACCAAACATCGGGAAGTTCATGTGAGAGAAAAGCCTCTGCCACACCCTCCATCTCTGTATTGCCCTGAGAACCCACATAAGGGAAAGACTGATGAATTTAGGAAAACTTTTTGATGATGATCTTCTCTTCCTAAATGCCCCCTTAGCCATTTTACCCTAATCTCATTCTTGGAAGCAATCTTTTCTTAGCTATGAAGTGTAATTTCCAAGATAAACTTGAGAATATAAATAAACTAAACACTTTCATCCTGTTCTCACCTCTGCCTCTAACTTCATCAGTCTGTTCCTCCTCTTCTGTGTGTCTCAGCTTCTCCTTTAAGAATGTGGCGAAGATACATTGTCCGAGTTCAGAATGAAATACCCTTCAGTACATTGAGGCCACTGCAACTGGATTGCTAAGTTCGGCAACATTCAAGATCTTCCATATGGGTGAGAGTTGTCTTCAAGGATATAAAAAATGCCAAAAGTTTTTTTGTTGTTATTACCTACTCCGTGAGATCCTGGATGAAACTTGATAACAGATTTTCACATTTAGATAGAACTTTGAGGAGTCTGATCGGAACTACATCAAATCCATCTTGGTTTTGAAGAGATCCACAAAAGAAGAGTGAAGATTTTATAGCTTTCCTCAGAAGTCTGTTACAGTGTACCTTTTTTTTTTTTTTGTCAAGAAGTTGATCCAAATAGCTAGGGCAGAATCCCTGTATTGTCACTGAAATCCTGTTACTAATGCCTTGCCCTCCATGGAGGTGAGAAGCAATTGATCACCCCTCATCCTTTTCTGTACCCGAAGACTTAAATCCCCTGGCTGCTGCTTCTCCAGCCTATAAGTAATCCCAGGTCCTCCTACTGGTTCTCATAGCCCTGGACTTGCCTCCCGATGGTCTGGGGGGTCCTGGCGGGCT
注意事项：该产品仅供科研实验使用，未经允许不得用于其它用途！以实际收货产品说明书为准，网站说明书仅供参考。
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文献和实验相关实验
XL PCR Amplification of Long Targets from Genomic DNA
Long polymerase chain reaction (PCR)>(1 5 ), specifically, XL PCR (Extra-Long Polymerase Chain Reaction), has enabled amplification of expanded trinucleotide repeats of the neuromuscular disease myotonic dystrophy (6 ) a 9-kb HIV
水平，也包括蛋白表达水平的缺失. 研究表明，在功能基因组研究中，功能缺失(loss of function，LOF)策略具有特殊重要地位. 1 反义寡脱氧核糖核苷酸和反义RNA 反义分子包括反义寡脱氧核糖核苷酸 (oligodeoxynucl-eotide，ODN)和反义RNA(antisense RNA)等. 反义技术(antisense technique)是指反义DNA/反义RNA分子作用及其机制研究的技术. 他是通过以碱基互补配对方式结合，抑制、封闭或破坏目的基因结构及其表达的核酸
5’和3’序列。每个GeneRacer™试剂盒中包含CIP、TAP、RNA连接酶、SuperScriptⅡ™逆转录酶等酶类及其缓冲液，GeneRacer™寡聚RNA，GeneRacer™ Oligo dT引物，GeneRacer™ PCR引物及RNase抑制剂。同时试剂盒中还包括S.N.A.P.凝胶纯化柱以纯化PCR产物，还有TOPO Cloning®试剂盒以进行下游测序。使用GeneRacer™试剂盒，您可以高效地得到全长的cDNA 5’和3’末端序列，而不必浪费时间筛选部分缺失或断裂的序列。
pCR-XL-TOPO-ZKSCAN2(human)(1点缺失)
￥580
