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- NM_031889.3(1点缺失);(1点缺失)
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- 2025年08月13日
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- 详细信息
- 文献和实验
- 技术资料
- 提供商：
上海博尔森生物科技有限公司
- 服务名称：
pCR4-TOPO-ENAM(1点缺失)(human)
- 规格：
干粉质粒
pCR4-TOPO-ENAM(1点缺失)(human)
[ 产品信息 ]
[ 质粒简介 ]
pCR4-TOPO-ENAM(1点缺失)(human)
[ 参考序列 ]
GGGACTAGTCCTGCAGGTTTAAACGAATTCGCCCTTTTGGCATTGGTTTCTTCTCAGGTTAAAGCTGTATTTTTCTTAAAGGCTTATAAAAATTTTGCTGAAAAAAATGTTGGTGCTTCGGTGCAGGCTTGGAACCTCTTTTCCTAAACTAGATAACTTGGTACCAAAAGGCAAAATGAAGATTCTCCTGGTCTTTCTAGGGCTTCTTGGTAATTCTGTTGCTATGCCAATGCACATGCCCCGAATGCCTGGATTTAGCAGTAAAAGTGAGGAGATGATGCGGTATAATCAATTCAACTTTATGAACGGCCCACATATGGCACACCTGGGGCCCTTCTTTGGAAACGGTCTCCCTCAGCAATTTCCACAGTACCAGATGCCCATGTGGCCTCAGCCACCACCCAACACATGGCATCCACGGAAATCCTCAGCACCCAAACGTCATAACAAGACTGATCAGACCCAAGAAACCCAGAAACCCAACCAGACTCAGTCAAAAAAGCCACCACAAAAGCGGCCTTTGAAGCAGCCATCACATAATCAACCTCAGCCCGAAGAGGAAGCTCAACCCCCTCAGGCATTCCCACCATTTGGAAATGGGCTATTCCCCTATCAACAACCACCATGGCAAATTCCACAGAGGTTACCACCACCAGGTTATGGACGCCCACCAATCAGCAATGAAGAAGGGGGGAATCCTTACTTTGGATATTTTGGATATCATGGCTTTGGGGGTCGCCCTCCTTATTATTCAGAAGAAATGTTTGAACAAGATTTTGAAAAACCCAAAGAAGAAGATCCTCCTAAAGCAGAAAGTCCAGGCACAGAACCCACAGCTAATTCAACAGTCACTGAGACGAATTCTACCCAACCAAATCCTAAAGGGAGTCAGGGAGGAAATGACACCAGCCCCACAGGAAACAGTACCCCAGGACTAAACACTGGGAACAACCCTCCAGCTCAAAATGGGATTGGCCCACTCCCTGCAGTCAACGCTTCAGGCCAGGGAGGGCCAGGAAGTCAAATCCCATGGAGACCAAGTCAGCCAAATATTCGTGAAAATCATCCATATCCTAATATAAGAAATTTTCCTTCAGGAAGACAGTGGTATTTCACTGGTACTGTCATGGGGCACAGACAGAATAGGCCTTTTTACAGAAATCAACAAGTTCAAAGGGGTCCTCGGTGGAACTTCTTTGCTTGGGAACGTAAACAAGTAGCTCGTCCAGGAAATCCAGTTTATCACAAAGCTTACCCTCCTACTTCAAGAGGCAATTATCCCAATTATGCAGGAAATCCAGCAAATCTCAGAAGAAAGCCTCAGGGGCCAAATAAACACCCTGTAGGAACTACTGTTGCCCCACTGGGTCCCAAACCTGGCCCTGTTGTTCGCAATGAAAAAATCCAAAATCCAAAGGAGAAGCCCCTGGGTCCAAAAGAACAAATAATAGTTCCTACAAAGAATCCAACCAGCCCCTGGAGAAACTCTCAACAGTATGAAGTTAATAAATCAAATTATAAACTGCCTCACTCTGAGGGTTATATGCCAGTCCCAAATTTTAATTCTGTTGATCAACATGAAAACTCCTATTACCCAAGAGGAGATTCCAGAAAAGTCCCAAATTCTGATGGACAAACCCAAAGCCAGAATTTGCCCAAAGGGATTGTTTTAGGGTCAAGAAGGATGCCATATGAATCAGAAACTAATCAGTCAGAATTAAAGCACAGCTCATATCAGCCTGCTGTATACCCTGAGGAAATCCCTTCTCCTGCAAAAGAACATTTTCCTGCTGGAAGAAATACTTGGGACCACCAAGAAATCTCTCCACCTTTTAAGGAAGATCCAGGGAGGCAAGAAGAACATTTACCCCATCCTTCCCATGGTTCTAGAGGAAGTGTTTTCTACCCTGAATATAACCCATATGATCCCAGGGAAAACTCACCATACCTTAGAGGCAATACATGGGATGAGAGAGATGATTCTCCCAATACTATGGGGCAAAAAGAAAGTCCACTCTACCCCATAAATACCCCAGACCAGAAGGAGATAGTCCCTTATAATGAAGAGGACCCAGTTGATCCAACTGGAGATGAAGTCTTTCCTGGACAAAATAGATGGGGTGAAGAGTTGAGCTTCAAAGGAGGCCCAACAGTTAGGCACTATGAAGGTGAACAATATACCTCAAATCAGCCAAAGGAATATCTTCCCTATTCTTTAGATAATCCATCAAAACCAAGGGAGGATTTTTATTACAGTGAATTTTACCCATGGAGCCCGGATGAGAATTTTCCATCATATAATACAGCTTCTACTATGCCACCACCTATAGAGAGCAGGGGCTACTACGTTAATAATGCCGCTGGACCAGAAGAAAGCACTCTATTTCCTTCACGGAATTCCTGGGACCACAGGATACAAGCCCAAGGGCAGAGAGAAAGAAGGCCGTATTTTAACAGAAATATCTGGGATCAGGCAACACATTTACAAAAAGCCCCAGCTAGGCCACCAGACCAGAAAGGTAACCAGCCCTATTACAGTAACACCCCAGCTGGGCTTCAGAAAAATCCAATATGGCATGAAGGTGAGAATTTGAACTATGGCATGCAAATAACTAGGATGAATTCTCCAGAGAGAGAACATTCATCTTTCCCTAACTTCATCCCACCAAGTTACCCATCAGGTCAAAAAGAAGCACATTTATTTCACCTAAGCCAGAGAGGCTCTTGCTGTGCTGGTAGCTCCACAGGGCCCAAGGACAATCCACTAGCTCTACAAGACTACACTCCATCCTATGGTCTTGCACCTGGGGAGAACCAAGACACCAGTCCTCTGTATACAGACGGTAGTCATACCAAGCAGACAAGAGATATCATCTCCCCAACAAGCATCCTACCAGGCCAAAGAAACAGCTCAGAGAAGAGGGAAAGCCAAAACCCTTTTAGAGATGATGTGTCCACGCTGAGGAGGAACACACCATGTTCTATAAAGAATCAACTGGGCCAAAAGGAAATTATGCCCTTTCCTGAAGCCAGTTCCCTTCAATCAAAGAATACACCTTGTCTCAAAATGATCTTGGAGGAGATGGGAACAACATTCTGGAACAAGTTTTTGAAGACAACCAGCTCAATGAAAGAACTGTTGACCTTACTCCTGAGCAGCTTGTTATTGGTACACCTGATGAAGGCTCCAATCCAGAAGGCATCCAAAGTCAAGTCCAAGAAAATGAGAGTGAGAGGCAACAGCAAAGACCATCTAACATTCTGCATTTGCCATGCTTTGGCTCCAAATTAGCAAAGCATCACTCTTCCACCACCGGAACTCCATCTAGCGATGGAAGGCAAAGCCCATTTGATGGGGATTCAATTACGCCTACTGAAAATCCTAACACATTGGTTGAGTTAGCTACTGAGGAACAATTTAAGAGTATAAATGTAGACCCACTTGATGCAGATGAACACAGTCCATTTGAATTCCTTCAAAGAGGGACCAATGTACAGGACCAGGTACAAGACTGCTTACTACTTCAGGCCTAGGGGTTATCCAACCAAGCATTCTTGGGGAAAGAGAAATCACTGAACCAAGGAAGGGCGAATTCGCGGCCGCTAAA
注意事项：该产品仅供科研实验使用，未经允许不得用于其它用途！以实际收货产品说明书为准，网站说明书仅供参考。
[ 产品信息 ]
| 名称 | pCR4-TOPO-ENAM(1点缺失)(human) | 别称 | NM_031889.3(1点缺失);(1点缺失) |
|---|---|---|---|
| 基因长 | 3429bp | 质粒宿主 | 大肠杆菌 |
| 质粒用途 | 基因模板 | 片段类型 | cDNA |
| 片段物种 | 人 | 原核抗性 | Amp |
| 货号: | BES226186PVE | 荧光标记 | |
| 启动子 | 复制子 | pUC | |
| 感受态 | DH5a | 温度 | 37度 |
| 背景载体 | 正向引物 | M13F：TGTAAAACGACGGCCAGT | |
| 反向引物 | M13R：CAGGAAACAGCTATGACC | 拷贝数 |
pCR4-TOPO-ENAM(1点缺失)(human)
[ 参考序列 ]
GGGACTAGTCCTGCAGGTTTAAACGAATTCGCCCTTTTGGCATTGGTTTCTTCTCAGGTTAAAGCTGTATTTTTCTTAAAGGCTTATAAAAATTTTGCTGAAAAAAATGTTGGTGCTTCGGTGCAGGCTTGGAACCTCTTTTCCTAAACTAGATAACTTGGTACCAAAAGGCAAAATGAAGATTCTCCTGGTCTTTCTAGGGCTTCTTGGTAATTCTGTTGCTATGCCAATGCACATGCCCCGAATGCCTGGATTTAGCAGTAAAAGTGAGGAGATGATGCGGTATAATCAATTCAACTTTATGAACGGCCCACATATGGCACACCTGGGGCCCTTCTTTGGAAACGGTCTCCCTCAGCAATTTCCACAGTACCAGATGCCCATGTGGCCTCAGCCACCACCCAACACATGGCATCCACGGAAATCCTCAGCACCCAAACGTCATAACAAGACTGATCAGACCCAAGAAACCCAGAAACCCAACCAGACTCAGTCAAAAAAGCCACCACAAAAGCGGCCTTTGAAGCAGCCATCACATAATCAACCTCAGCCCGAAGAGGAAGCTCAACCCCCTCAGGCATTCCCACCATTTGGAAATGGGCTATTCCCCTATCAACAACCACCATGGCAAATTCCACAGAGGTTACCACCACCAGGTTATGGACGCCCACCAATCAGCAATGAAGAAGGGGGGAATCCTTACTTTGGATATTTTGGATATCATGGCTTTGGGGGTCGCCCTCCTTATTATTCAGAAGAAATGTTTGAACAAGATTTTGAAAAACCCAAAGAAGAAGATCCTCCTAAAGCAGAAAGTCCAGGCACAGAACCCACAGCTAATTCAACAGTCACTGAGACGAATTCTACCCAACCAAATCCTAAAGGGAGTCAGGGAGGAAATGACACCAGCCCCACAGGAAACAGTACCCCAGGACTAAACACTGGGAACAACCCTCCAGCTCAAAATGGGATTGGCCCACTCCCTGCAGTCAACGCTTCAGGCCAGGGAGGGCCAGGAAGTCAAATCCCATGGAGACCAAGTCAGCCAAATATTCGTGAAAATCATCCATATCCTAATATAAGAAATTTTCCTTCAGGAAGACAGTGGTATTTCACTGGTACTGTCATGGGGCACAGACAGAATAGGCCTTTTTACAGAAATCAACAAGTTCAAAGGGGTCCTCGGTGGAACTTCTTTGCTTGGGAACGTAAACAAGTAGCTCGTCCAGGAAATCCAGTTTATCACAAAGCTTACCCTCCTACTTCAAGAGGCAATTATCCCAATTATGCAGGAAATCCAGCAAATCTCAGAAGAAAGCCTCAGGGGCCAAATAAACACCCTGTAGGAACTACTGTTGCCCCACTGGGTCCCAAACCTGGCCCTGTTGTTCGCAATGAAAAAATCCAAAATCCAAAGGAGAAGCCCCTGGGTCCAAAAGAACAAATAATAGTTCCTACAAAGAATCCAACCAGCCCCTGGAGAAACTCTCAACAGTATGAAGTTAATAAATCAAATTATAAACTGCCTCACTCTGAGGGTTATATGCCAGTCCCAAATTTTAATTCTGTTGATCAACATGAAAACTCCTATTACCCAAGAGGAGATTCCAGAAAAGTCCCAAATTCTGATGGACAAACCCAAAGCCAGAATTTGCCCAAAGGGATTGTTTTAGGGTCAAGAAGGATGCCATATGAATCAGAAACTAATCAGTCAGAATTAAAGCACAGCTCATATCAGCCTGCTGTATACCCTGAGGAAATCCCTTCTCCTGCAAAAGAACATTTTCCTGCTGGAAGAAATACTTGGGACCACCAAGAAATCTCTCCACCTTTTAAGGAAGATCCAGGGAGGCAAGAAGAACATTTACCCCATCCTTCCCATGGTTCTAGAGGAAGTGTTTTCTACCCTGAATATAACCCATATGATCCCAGGGAAAACTCACCATACCTTAGAGGCAATACATGGGATGAGAGAGATGATTCTCCCAATACTATGGGGCAAAAAGAAAGTCCACTCTACCCCATAAATACCCCAGACCAGAAGGAGATAGTCCCTTATAATGAAGAGGACCCAGTTGATCCAACTGGAGATGAAGTCTTTCCTGGACAAAATAGATGGGGTGAAGAGTTGAGCTTCAAAGGAGGCCCAACAGTTAGGCACTATGAAGGTGAACAATATACCTCAAATCAGCCAAAGGAATATCTTCCCTATTCTTTAGATAATCCATCAAAACCAAGGGAGGATTTTTATTACAGTGAATTTTACCCATGGAGCCCGGATGAGAATTTTCCATCATATAATACAGCTTCTACTATGCCACCACCTATAGAGAGCAGGGGCTACTACGTTAATAATGCCGCTGGACCAGAAGAAAGCACTCTATTTCCTTCACGGAATTCCTGGGACCACAGGATACAAGCCCAAGGGCAGAGAGAAAGAAGGCCGTATTTTAACAGAAATATCTGGGATCAGGCAACACATTTACAAAAAGCCCCAGCTAGGCCACCAGACCAGAAAGGTAACCAGCCCTATTACAGTAACACCCCAGCTGGGCTTCAGAAAAATCCAATATGGCATGAAGGTGAGAATTTGAACTATGGCATGCAAATAACTAGGATGAATTCTCCAGAGAGAGAACATTCATCTTTCCCTAACTTCATCCCACCAAGTTACCCATCAGGTCAAAAAGAAGCACATTTATTTCACCTAAGCCAGAGAGGCTCTTGCTGTGCTGGTAGCTCCACAGGGCCCAAGGACAATCCACTAGCTCTACAAGACTACACTCCATCCTATGGTCTTGCACCTGGGGAGAACCAAGACACCAGTCCTCTGTATACAGACGGTAGTCATACCAAGCAGACAAGAGATATCATCTCCCCAACAAGCATCCTACCAGGCCAAAGAAACAGCTCAGAGAAGAGGGAAAGCCAAAACCCTTTTAGAGATGATGTGTCCACGCTGAGGAGGAACACACCATGTTCTATAAAGAATCAACTGGGCCAAAAGGAAATTATGCCCTTTCCTGAAGCCAGTTCCCTTCAATCAAAGAATACACCTTGTCTCAAAATGATCTTGGAGGAGATGGGAACAACATTCTGGAACAAGTTTTTGAAGACAACCAGCTCAATGAAAGAACTGTTGACCTTACTCCTGAGCAGCTTGTTATTGGTACACCTGATGAAGGCTCCAATCCAGAAGGCATCCAAAGTCAAGTCCAAGAAAATGAGAGTGAGAGGCAACAGCAAAGACCATCTAACATTCTGCATTTGCCATGCTTTGGCTCCAAATTAGCAAAGCATCACTCTTCCACCACCGGAACTCCATCTAGCGATGGAAGGCAAAGCCCATTTGATGGGGATTCAATTACGCCTACTGAAAATCCTAACACATTGGTTGAGTTAGCTACTGAGGAACAATTTAAGAGTATAAATGTAGACCCACTTGATGCAGATGAACACAGTCCATTTGAATTCCTTCAAAGAGGGACCAATGTACAGGACCAGGTACAAGACTGCTTACTACTTCAGGCCTAGGGGTTATCCAACCAAGCATTCTTGGGGAAAGAGAAATCACTGAACCAAGGAAGGGCGAATTCGCGGCCGCTAAA
注意事项：该产品仅供科研实验使用，未经允许不得用于其它用途！以实际收货产品说明书为准，网站说明书仅供参考。
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文献和实验相关实验
5’和3’序列。每个GeneRacer™试剂盒中包含CIP、TAP、RNA连接酶、SuperScriptⅡ™逆转录酶等酶类及其缓冲液，GeneRacer™寡聚RNA，GeneRacer™ Oligo dT引物，GeneRacer™ PCR引物及RNase抑制剂。同时试剂盒中还包括S.N.A.P.凝胶纯化柱以纯化PCR产物，还有TOPO Cloning®试剂盒以进行下游测序。使用GeneRacer™试剂盒，您可以高效地得到全长的cDNA 5’和3’末端序列，而不必浪费时间筛选部分缺失或断裂的序列。
水平，也包括蛋白表达水平的缺失. 研究表明，在功能基因组研究中，功能缺失(loss of function，LOF)策略具有特殊重要地位. 1 反义寡脱氧核糖核苷酸和反义RNA 反义分子包括反义寡脱氧核糖核苷酸 (oligodeoxynucl-eotide，ODN)和反义RNA(antisense RNA)等. 反义技术(antisense technique)是指反义DNA/反义RNA分子作用及其机制研究的技术. 他是通过以碱基互补配对方式结合，抑制、封闭或破坏目的基因结构及其表达的核酸
的克隆方法将目的基因克隆进入门载体。 （2）混合包含目的基因的入门克隆和合适的目的载体以及Gateway LR Clonase酶，构建表达克隆。（表达克隆用来在合适的宿主中进行蛋白的表达和分析。） 有几种方法可以构建Gateway入门克隆。无论您选择何种方法，创建的入门克隆都是准备用来与各种目的载体进行重组。 （1）PCR克隆（定向TOPO克隆至入门载体或与供载体B×P重组） （2）限制性内切酶消化和连接进入入门载体 （3）使用pCMV•SPORT6或pEXP-AD502*构建
pCR4-TOPO-ENAM(1点缺失)(human)
￥680
