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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
705-067-003
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Biotin-SP-conjugated AffiniPure® Fab Fragment Donkey Anti-Goat IgG (H+L)
- 规格:
0.5ml
产品简介:
品牌:Jacksonimmuno
货号:705-067-003
产品名称:Biotin-SP-conjugated AffiniPure® Fab Fragment Donkey Anti-Goat IgG (H+L)
https://www.jacksonimmuno.com/catalog/products/705-067-003
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文献和实验多余液体;加A蛋白一抗50μL(1:100),4℃下过夜;PBS洗3次,每次5min,加荧光标记的A蛋白二抗 [Alexa Fluor® 488 donkey anti-goat IgG (H+L)] 50μL(1:200),室温下放置60min;PBS冲洗3次,加B蛋白一抗50μL(1:500)37℃下放置1h,PBS洗3次,每次5min;加荧光标记的B蛋白二抗[(Anti-Rabbit IgG (H+L), F(ab')2 Fragment)] 50μL(1:200),置于室温下 60 min
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
Immunohistochemisty-Fluorescence Protocol
: If using R&D Systems biotinylated antibody skip steps 9 and 10 and continue. Incubate each cell spot on slides for 30 minutes at room temperature with 15 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted
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