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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
515-095-062
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Fluorescein (FITC)-conjugated AffiniPure® Sheep Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs Sr Prot)
- 规格:
1.5mg
产品简介:
品牌:Jacksonimmuno
货号:515-095-062
产品名称:Fluorescein (FITC)-conjugated AffiniPure® Sheep Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs Sr Prot)
https://www.jacksonimmuno.com/catalog/products/515-095-062
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
min。②荧光素的准备 根据欲标记的蛋白质总量,按每毫克免疫球蛋白加0.01mg荧光色素,用分析天平准确称取所需的异硫氰酸荧光素粉末。也可用下述公式计算出免疫球蛋白、荧光素的量,还可以算出需加缓冲液的量。a.蛋白溶液:含量Amg/m1;容积Bml。b.总蛋白量(AXB)=Crag。c.C/20~C/10=Dmg(如蛋白含量低于20mg/ml,用C/10;如高于20mg/ml,用C/20)。d.荧光素FITC的量:(1/50~2/100)XC=Emg。e.巳0.5mol/L(pH9.5)碳酸盐缓冲
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