- ￥2578
- Jacksonimmuno
- 美国
- 315-055-008
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 目录编号：
315-055-008
- 库存：
详询
- 供应商：
广州市左克生物
- 浓度：
见包装
- 抗体英文名：
Alkaline Phosphatase-conjugated AffiniPure® Rabbit Anti-Mouse IgG, Fcγ Fragment Specific
- 规格：
1ml
产品简介:
品牌：Jacksonimmuno
货号：315-055-008
产品名称：Alkaline Phosphatase-conjugated AffiniPure® Rabbit Anti-Mouse IgG, Fcγ Fragment Specific
https://www.jacksonimmuno.com/catalog/products/315-055-008
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
The methods described in this article are relative to the use of a positive cloning/screening recombinant system for the generation in Escherichia coli of foreign proteins fused to a highly active bacterial alkaline phosphatase (PhoA) variant
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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