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- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
315-035-008
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Peroxidase-conjugated AffiniPure® Rabbit Anti-Mouse IgG, Fcγ Fragment Specific
- 规格:
1.5ml
产品简介:
品牌:Jacksonimmuno
货号:315-035-008
产品名称:Peroxidase-conjugated AffiniPure® Rabbit Anti-Mouse IgG, Fcγ Fragment Specific
https://www.jacksonimmuno.com/catalog/products/315-035-008
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文献和实验Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
immunoglobulin G subclass 1 fragment crystallizable (IgG1 Fc), by native chemical ligation. The glycosylated IgG1 Fc is expressed in a glycosylation-deficient yeast strain. The N-terminal cysteine is generated by the endogenous yeast protease Kex2 in the yeast
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
by a two- or three-step procedure. The method can insert any fragment into small or large episomes, without the need of an antibiotic selection gene. We have humanized the mouse luteinizing hormone receptor gene (Lhcgr ) by inserting a ~55 kb fragment from a BAC clone
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