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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
315-005-049
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
AffiniPure® Rabbit Anti-Mouse IgM, µ Chain Specific (min X Hu Sr Prot)
- 规格:
1.5mg
产品简介:
品牌:Jacksonimmuno
货号:315-005-049
产品名称:AffiniPure® Rabbit Anti-Mouse IgM, µ Chain Specific (min X Hu Sr Prot)
https://www.jacksonimmuno.com/catalog/products/315-005-049
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文献和实验the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Chromosome Conformation Capture (3C) (PROT05)
-X to a final concentration of 1.8% (180µl of 20% Triton-X) to sequester the SDS. Incubate 1 hour at 37°C with shaking; Count the nuclei using a haemocytometer; Use a 1x106 nuclei aliquot (~15µg) for digestion. Digest overnight at 37°C
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