- ￥3820
- Jacksonimmuno
- 美国
- 300-055-245
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 目录编号：
300-055-245
- 库存：
详询
- 供应商：
广州市左克生物
- 浓度：
见包装
- 抗体英文名：
Alkaline Phosphatase-conjugated AffiniPure® Rabbit Anti-GFP
- 规格：
0.1ml
产品简介:
品牌：Jacksonimmuno
货号：300-055-245
产品名称：Alkaline Phosphatase-conjugated AffiniPure® Rabbit Anti-GFP
https://www.jacksonimmuno.com/catalog/products/300-055-245
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
Secondary antibody: Goat anti-mouse alkaline phosphatase conjugated - Biorad #170-6520. Substrate: ECF chemifluorescent substrate - Amersham #RPN5785. Mix substrate with accompanying buffer as per manufacturer’s
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